| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Methanosphaera cuniculi | GCAAGCGAACTTAAATGGGGA | TTTCCTGCACGAATGTTGGT | 59.18 | 58.31 | 176 | 106 |
100.00%
|
100.00%
|
| Primer 132 | Methanosphaera cuniculi | TGGGGACAACGTCAATTCAG | TTTCCTGCACGAATGTTGGT | 58.11 | 58.31 | 161 | 106 |
100.00%
|
100.00%
|
| Primer 133 | Methanosphaera cuniculi | AGCATCAAACTGGGCTGCT | AGGGTCAGTTGGTGTTTCTGG | 59.92 | 60.13 | 217 | 106 |
100.00%
|
100.00%
|
| Primer 134 | Methanosphaera cuniculi | AGCATCAAACTGGGCTGCT | GGGTCAGTTGGTGTTTCTGGA | 59.92 | 60.13 | 216 | 106 |
100.00%
|
100.00%
|
| Primer 135 | Methanosphaera cuniculi | TGACAATGGCAGTATGGGCT | ACAGCTCCTGGTGCTAGAAA | 59.37 | 58.65 | 207 | 106 |
100.00%
|
100.00%
|
| Primer 136 | Methanosphaera cuniculi | GTGATGAGGCAAAAGCACGAA | TGCCCATGCATCACCTTGA | 59.73 | 59.62 | 170 | 106 |
100.00%
|
100.00%
|
| Primer 137 | Methanosphaera cuniculi | TGGCCTGCTGTTTTGGGA | ACAACAAGCAAACCAACAGGT | 59.39 | 59.10 | 224 | 106 |
100.00%
|
100.00%
|
| Primer 138 | Methanosphaera cuniculi | TGGCCTGCTGTTTTGGGAA | ACAACAAGCAAACCAACAGGT | 60.08 | 59.10 | 224 | 106 |
100.00%
|
100.00%
|
| Primer 139 | Methanosphaera cuniculi | ATGGCCTGCTGTTTTGGGA | ACAACAAGCAAACCAACAGGT | 59.84 | 59.10 | 225 | 106 |
100.00%
|
100.00%
|
| Primer 140 | Methanosphaera cuniculi | ACCCTGAACAACTCCGCT | AGGACATGTTGCTACAGCCA | 58.44 | 59.31 | 176 | 106 |
100.00%
|
100.00%
|